Abstract

Simeon Oloni KOTCHONI1, Emma Wanjiru GACHOMO2, Eriola BETIKU3, and Olusola OlusojiSHONUKAN4

Many methods have been used to isolate plasmid DNA, but some of them are time consuming especially when extracting a large number of samples. Here, we developed a rapid protocol for plasmid DNA extraction based on the alkaline lysis method of plasmid preparation (extraction at pH 8.0). Using this new method, a good plasmid preparation can be made in approximately one hour. The plasmids are suitable for any subsequent molecular applications in the laboratory. By applying the recommendations to avoid contaminations and to maximize the plasmid yield and quality during extraction,, this protocol could be a valuable reference especially when analyzing a large number of samples.