Abstract

Wu Yi, Li Peng and Wang Xueqian

Kidney transplantation to treat end-stage renal disease has evolved rapidly from the first successful transplantations to the current widespread use of grafts from both cadaveric and living donors. But acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate possible mechanisms, we describe a comparison between different proteins expression profile of acute rejection and the controls. Through two-dimensional difference gel electrophoresis, mass spectral techniques microarray analysis and quantitative real-time RT-PCR confirmation, marked change in expression level of 37 protein spots was observed in allograft rejection group. Among them, expression levels of 24 protein spots were up-regulated, while expression levels of 13 protein spots were down-regulated. Among 37 protein spots, 29 obtained satisfactory peptide mass fingerprinting. We identified 6 proteins in acute rejection after renal transplantation. Then, we investigated PDIA3 levels in serum and transplanted kidney, PDIA3 mRNA and protein expression level. Our data thus indicate that PDIA3 might be potentially involved into the occurrence and development of acute rejection response in renal transplantation.