Abstract

M. Rajalakshmi, J. Eliza, Cecilia Edel Priya, Nirmala A and P. Daisy*

The oral administration of various extracts (hexane, ethyl acetate and methanol) of Tinospora cordifolia stem (TCS) were found to have potent antidiabetic activity that reduces blood sugar level in streptozotocin-(STZ) induced diabetic rats. In this study, the chronic (100 days) antihyperglycemic effect of the extracts at a dose of 250 mg/kg b.w.p.d of TCS were investigated. Insulin was used as a reference drug at a dose of 3 I.U/kg.b.w.p.d. Fasting blood glucose, glycosylated hemoglobin (HBA1C), serum insulin, C- peptide and liver enzymes levels were evaluated in normal, diabetic and treated rats. Supplementation of methanol extract significantly reduces the fasting blood glucose level when compared to other 2 extracts. Moreover this supplementation significantly decreases the glycosylated hemoglobin level as compare to diabetic control (p < 0.001), reduced glucokinase and increased glucose-6- phoaphatase activity were reversed significantly by the treatment of TCS methanol extract in respect to diabetic group. In the TCS treated groups, the insulin and C-peptide levels were improved which shows the regeneration of -cell which secretes insulin, histopathological studies of pancreas of TCS methanol extract treated groups substantiate the regenerating capacity of extract.