Abstract

Sidjui Sidjui, Lazare,* , Melong, Raduis , Nnanga, Nga , Toghueo Kouipou, Rufin Marie, Zeuko�??o Menkem, Elisabeth , Sikadeu, Sandrine , Guedje, Nicole Marie , and Folefoc Ngosong, Gabriel

This study was conducted to evaluate the antimicrobial and cytotoxicity activities of extracts from root and stem barks of Pseudospondias microcarpa. The root and stem barks were extracted successively by nHexane, Methylene chloride, ethyl acetate, Methanol, Methanol-Methylene Chloride, and ethanol-water. The antimicrobial activity was evaluated by disc diffusion method against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Candida albicans, Streptomyces viridochromogenes Tü 57 and Mucormiehei. Cytotoxicity activity of extracts was measured by Brine shrimp lethality bioassay and their phytochemical screening was studied.The inhibition zone diameter of the nineteen extracts ranged from 0-15 mm. PMF4 and PMF7 with 15 mm showed moderate activity on Staphylococcus aureus. PM11 and PMF6 showed 14 mm on Bacillus subtilis, Escherichia coli and Candida albicans respectively. At 24 h, the percentage of lethality vary from 0-21 % with PMF2 and PM3 having 19 and 21 % respectively. At 32 h, percentage varies from 0-56 % with PMF1, PMF2 and PM3 exhibited 27, 38 and 56 % respectively. Phytochemical screening revealed the presence of sterols, alkaloids, flavonoids, cardiac glycosides, saponins, quinones, glycosides, polyphenols, triterpenes, anthocyanins, anthraquinons, tannins and coumarins. This work revealed the antimicrobial potentials of this plant supporting their traditional use.