Biolistic transformation of Saccharomyces cerevisiae with β-glucosidase gene from Cellulomonas biazotea

S. Parvez; Z. Mukhtar; F. Rashid; M.I. Rajoka

Biolistic transformation of Saccharomyces cerevisiae with β-glucosidase gene from Cellulomonas biazotea

Abstract

S. Parvez*, Z. Mukhtar, F. Rashid, M.I. Rajoka

A β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae. Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicating that the gene is expressed in the bombarded yeast.

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