Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhI.2)

Howard RL

Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhI.2)

Abstract

Howard RL

The Phanerochaete chrysosporium ME446 cbhI.2 cDNA was cloned and expressed in Escherichia coli using a pET system. Pulse-labelling revealed the expression of an induced protein of approximately 50- 60 kDa that reacted with anti-P. chrysosporium ME446 CBH antibodies. The expressed protein remained undegraded in vivo for 135 min but was biologically inactive in vitro.

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