Comparative analysis of genomic DNA isolation procedures; hexa decyltrimethylammonium bromide (CTAB) and liquid detergent (morning fresh®
) methods from Samsorg 41 (icsv 400)

Inuwa H. M.; Aimola I. A; Inuwa L.

Comparative analysis of genomic DNA isolation procedures; hexa decyltrimethylammonium bromide (CTAB) and liquid detergent (morning fresh® ) methods from Samsorg 41 (icsv 400)

Abstract

Inuwa H. M., Aimola I. A* and Inuwa L.

Genomic DNA was prepared from germinating seeds of a variety of sorghum in Nigeria (Samsorg 41) using two different isolation protocols (CTAB and morning fresh® method). Highest quantity of DNA was obtained from Samsorg 41 using the morning fresh® method. Genomic DNA obtained using this method had a much lower purity as compared with that obtained using the CTAB method. Both methods produced genomic DNA of high integrity with minimum shearing.

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