Abstract

Saman Mahdavi1*, Taghi Zahraei Salehi 2, Rasoul Madani3and Hadi Keyvanfar4.

Mycoplasma agalactiae is one of the chief factors causative contagious agalactiae in sheep and goats that involves huge geographical regions such as Iran and causes economic losses. In this disease many clinical signs may be observed for example: mastitis, agalactiae, Keratoconjuctivitis, arthritis, lameness and sometimes abortion and Pneumonia. Cytoplasmic membrane Protein 40 KDa (P40) in this bacterium is a strong immunogenic protein, major virulence factor and is specific for M. agalactiae. Isolated strains from Iran were considered molecularly and genetically for identification of gene concerning this protein. The P40 gene of isolated M. agalactiae strains in Iran was amplified with PCR technique and for further studies this fragment were cloned and sequenced. In comparative study of P40 gene sequence of isolated strains in Iran with other strains in gene bank with the use of BLAST program showed us the homology (Identities 100%) of Lorestan strain with French strains 4258, 4021 and Spanish strain PG2 and as for Taleghan and Shiraz strains the most homology (Identities 99%) was achieved with French Strains 4258, 4021 and Spanish strains PG2, 6968, 5225 and Swiss strain 7375 and Italian strain 9. The Least homology (Identities 84%) for all three strains was reported with French strain 4212.