Abstract

Owunmi D. Amstrong , Diedeji Atamu , Orhomedia E. H. and Aruoture Destiny

The viability change of the probiotic organisms on the feed was analysed at 5 days interval for a period of 25 days to observe the effect of different storage temperatures on the viabilities change of probiotics (Lactobacillus brevis1, Lactobacillus plantarum, Pediococcus pentosaceus2) in the feed. The aim of this research was to study the survival of the probiotics during storage. Feed was sprayed with a suspension of 3.2 × 108 CFU/ml of probiotics and the viability of the cultured organisms was tested under 4 different temperatures (room temperature (22°C), 0, 4, and 8°C). After spraying, feed was kept at 37°C for 24 h and dried at room temperature prior to the test. The results demonstrated that a refrigeration temperature of 0°C led to highest viability of the organisms but the feed were subjected to chemical damage during freeze-thaw which invariably cause death of some of those organisms. Hence, fish feed incorporated with probiotic should be stored at 4°C. Organisms could not withstand room temperature for more than 15 days after which the viability of the organisms began to drop. Temperature is being considered as a critical factor influencing probiotic viability and survival during storage period.