Abstract

Zahoor. A. Kaloo*, Rukhsana Akhtar, Zafrul-Haq and B. A. Wafai

The present work as undertaken to study the effect of auxins and Cytokinins on the in vitro multiplication of nodal cuttings with axillary buds and runner tips with apical buds of Viola odorata a medicinal herb growing in Kashmir and western Himalayas. The growth medium used was MS (1962) basal medium supplemented with BAP, Kn and NAA at varying concentrations. The multiplication rate of shoots increased with increasing the concentration of BAP and Kn. However the optimum results were obtained on MS medium supplemented with a combination of 15 µm NAA and 10 µm BAP. Nodal segments showed better results than runner tips.