Abstract

Dipankar Ghosh1*, Susmita Santra, Sujit Dutta and Panchanan Pramanik

The study focused on effective inhibition of the agglutinability of human A and B type erythrocytes by sodium periodate-chloroacetic acid concerted action. Suppression of agglutinability was determined by using anti-A and anti-B antisera. Effective concentrations of sodium periodate were 6.8 and 2.7 mM, respectively for A and B type erythrocytes in presence of 4.5 mg.ml-1 chloroacetic acid in the reaction mixture. Some major biophysiological consequences of human erythrocytes including agglutination index, spontaneous red blood cell lysis percentage, zetapotential, morphology, surface functional groups (- OH, > C = O, -COOH) and semiquantitative oxygen uptake and phagocytic uptake were also analyzed after the chemical treatments. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.