Abstract

Xinhuai Zhao*, Yu Zhang and Dan Li

Lactobacillus bulgaricus or Lactobacillus helveticus was incubated in skim milk with addition of sodium citrate, calcium carbonate or sodium carbonate at 37°C for 24 h, whereas Bifidobacterium animalis or Bifidobacterium infantist was incubated in skim milk with addition of sodium Disoascorbate, sodium L-ascorbate or L-cysteine at 37°C for 24 h in an anaerobic chamber. Viable count of L. bulgaricus, L. helveticus, B. animalis or B. infantist in culture was enumerated by standard plate counting technique. The result showed that the viable count of L. bulgaricus or L. helveticus had an increase about 150 ~ 220%, B. animalis or B. infantist in culture had an increase about 150 ~ 190%. The effects of these chemicals that could eliminate acidic or oxidative stress for four probiotics during incubation in vitro were validated thereby, which implies that such technique might be a simple and effective approach for probiotic production in higher density.