Abstract

Tan FeiQuan, Fu ShuLan, Tang ZongXiang, Ren ZhengLong, Zhang HuaiQiong

3 sets of sibling 1BL.1RS translocation wheat lines (each set containing 2 sibling lines) derived from 3 different
F4 single plant. Between 2 sibling wheat lines, different resistance to powdery mildew was observed. In addition,
different bands in -gliadin mobility zone between 2 sibling wheat lines were also significantly observed by acid
polyacrylamide gel electrophoresis (A-PAGE). However, the nucleotide sequence of / -gliadin precursor gene
cloned from 2 sibling wheat lines were 100% similarity. Polymerase chain reaction (PCR) analysis using 180
wheat microsatellite markers dispersed on 7 homeologous groups of wheat indicated that little genetic variation
occurred in wheat A, B and D genomes of these sibling wheat lines. Structural variation of 1BL.1RS chromosome
arm was detected by PCR analysis. Different rye-specific repetitive DNA exhibited different variation. The
different structure of 1RS chromosome arms may be related to the genetic variation between the 2 sibling wheat
lines. The results lead us to think that the variation of 1RS arm within a species should be utilized in wheat
breeding program. To detect variation of repetitive DNA can provide better understanding of the genetic variation
of 1BL.1RS translocation and will be useful for utilization of 1RS arm in wheat breeding program.