Abstract

Shahedur Rahman*, Anowar Khasru Parvez and Rezwanul Islam

This study was undertaken to identify and quantify Escherichia coli from drinking water from the western part of Bangladesh. Fifty water samples were collected from different regions. The presence of E. coli was determined on the basis of gas formation in the test tubes after inoculating and incubating the water samples into the Lactose fermentation broth (LB). A certain amount of the accumulated gas was used to determine the quantity of the E. coli cell present in the water samples with the help of Minimum Probable Number (MPN) method. Then the confirmation test was done using Eosin Methylene Blue agar. Positive isolates were then subjected to the completed test (Gram staining). Among the 50 different water samples, 5 samples were found positive.