Abstract

Abdelmonem Messaoudi1*, Maher Gtari1, Abdellatif Boudabous1and Florian Wagenlehner 2

Identification and susceptibility to 8 selected antimicrobial agents of Klebsiella and Enterobacter bacterial species was studied in 15 marketed meat samples (chicken, turkey-hen, beef, sheep, pig, dromedary, ostrich, and fish). The isolates were identified with the API 20E system, resulting in 7 clusters: Enterobacter aerogenes (2 isolates), Enterobacter cloacae (6), Enterobacter sakazakii (3), Enterobacter spp (14), Klebsiella oxytoca (5), Klebsiella pneumoniae (2) and Klebsiella ornithinolytica (12). The identities of isolates identified as Enterobacter and Klebsiella spp. were confirmed by Amplified Ribosomal DNA Analysis (ARDRA), using AluI, MspI, RsaI restriction enzymes. Identification of isolates by ARDRA and API 20E system gave similar results with 90,2 % (44/51) of the collection. Susceptibility to antibiotics was evaluated by the disk method according to French recommendations (CA-SFM). Resistance rates were as follows: Ampicillin (62%), cefalotin (30%), tetracycline (14%), chloramphenicol 2%. aminoglycosides 0% and nalidixic acid 0%. Strains isolated from 'industrially reared animals' showed higher resistance rates than 'naturally reared' ones.