Abstract

Ayman Ashmaig, Alaa Hasan and Eisa El Gaali*

From 12 samples of gariss, a Sudanese traditionally fermented camel©s milk, 24 lactic acid bacteria were isolated and phenotypically characterized by biochemical tests and their ability to ferment different carbohydrates. Analysis of the observed data resulted in classifying the isolates into 11 groups, each group containing one or more isolates, 2 major groups were identified, the larger one composed of 7 isolates recognized as Lactobacillus plantarum (66.6% of the gariss isolates) and the second was composed of 6 isolates recognized as Lactococcus raffinolactis (33.3% of the gariss isolates). The remained minor groups were classified as Lactobacillus animalis, Lactobacillus brevis, Lactobacillus divergens, Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactococcus alimentarium, Lactobacillus sp. The genetic relationships of the isolates were determined by the random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR), the results demonstrated a distinction comparative genetic clusters and their pattern was greatly related to the clustering obtained with the API 38 CHL group identification.