Abstract

Hybrid rice, genetic purity, SDS-PAGE, fertility restorer genes.

For identification of rice hybrids and their parental lines, thirty populations produced from hybridization between a cytoplasmic male sterility (CMS) line (IR69625A), and each of the three restorer lines (Giza178R, Giza181R and Giza182R) during 2009, 2010 and 2011 seasons were used. Electrophoresis of proteins showed promising results in genetic purity determination of hybrids and parental lines. The presence or absence of a specific band was much useful for differentiating the highest from the lowest parents in restoring ability. Five markers linked to fertility restorer genes: M2, RM3425, RM258, RM1108 and RM5373 were screened on DNA templates from the CMS line and its three restorer lines. Only RM1108 marker was able to show polymorphism between the highest and lowest Giza181R parents. This marker could be assessed for identification and testing of seed genetic purity of restorer lines.