Abstract

Sang Un Park , Yong Kyoung Kim and Sook Young Lee *

The establishment of an efficient protocol for plant regeneration and micropropagation from leaf cultures of Rehmannia glutinosa L. is reported. The regenerated shoots obtained from leaf cultures on solid MS medium containing different concentractions of TDZ. The highest number of shoots per explant (2.1) and shoot growth (1.2 cm) was obtained on MS medium containing 1 mg/L TDZ. The addition of 0.1 mg/L NAA and 3g/L Gelrite in MS medium containing 1 mg/L TDZ substantially improved the shoot regeneration of R. glutinosa. The rooted plants were hardened and transferred to soil with a 73% survival rate. The continuous production of R. glutinosa regenerated plants could be used as a possible micropropagation system.