Abstract

Peter Kamau Ngure *, Willy Kiprotich Tonui , Johnstone Ingonga , Charles Mutai ,Elizabeth Kigondu , Zipporah Ng

The antileishmanial activity of extracts of Warburgia ugandensis (family Canellaceae), a known traditional therapy and one of the commonly used medicinal plants in Kenya was evaluated. Extracts of this plant were tested for possible antileishmanial activity in vitro. Different doses of hexane, dichloromethane, ethyl acetate and methanol extracts of W. ugandensis were tested against Leishmania major and Leishmania donovani promastigotes and amastigotes. The hexane extract had the best activity against L. major promastigotes and amastigotes with IC50 value of 9.95 for promastigotes and 8.65 for amastigotes and minimum inhibition concentrations of 62.5 µg/ml. The activity of the hexane extract on amastigotes was comparable (P > 0.05) to that of pentostam and amphotericin B. Similar results were obtained for L. donovani with IC50 values of 8.67 for promastigotes and 100-fold reduction of amastigotes in macrophage cultures. Warburgia ugandensis had lower levels of toxicities compared to pentostam and amphotericin B. This study scientifically demonstrates the potential of W. ugandensis in the treatment of leishmaniasis.