Abstract

Pir Bux Ghumro*, Maryam Shafique, Muhammad Ishtiaq Ali, Imran Javed, Bashir Ahmad , Asif Jamal, Naeem Ali and Abdul Hameed

Thermophilic bacteria produce commercially useful proteases, active at high temperature. Due to growing demand of proteases application in different industries, the present study was designed to isolate protease producing Bacillusstrains. Nine strains were isolated from three different ecological sources and qualitatively screened on skim milk agar. The Bacillusstrain PB1 showed 5.4 cm zone of hydrolysis. The screened protease producing strain was further identified on the basis of morphological, biochemical (API 20 and API 50 CHB) and 16 s rRNA molecular identification as Bacilluslicheniformis PB1 (Accession EU650317)