Abstract

Sirichai Adisakwattana *, Jeerasuk Moonrat, Supatra Srichaira , Chuliporn Chanasit, Hathaichanok Tirapongporn, Benjanut Chanathong, SathapornNgamukote, Kittana Mäkynenand Suwimol Sapwarobol

Grape Seed Extract (GSE) has been targeted to promote beneficial health effects, especially the prevention of obesity and hyperlipidemia. In present study, we investigated the mechanisms of GSE on inhibition of lipid digestion and absorption. GSE significantly inhibited pancreatic lipase and cholesterol esterase in dose-dependent manner with the IC50 values of 3.71 ± 0.03 and 27.27 ± 4.12 mg/mL, respectively. In addition, GSE also inhibited the formation of cholesterol micelles, and bound to bile acid. The oral administration of GSE significantly decreased serum triglyceride and cholesterol in rat fed high fat emulsion through inhibition of lipid digestion and absorption. GSE may be a feasible therapeutic strategy for prevention and treatment of patients with hyperlipidemia and obesity.