Abstract

Afiukwa, Ngozi Felicitas, Iroha Ifeanyichukwu Romanus*, Afiukwa Celestine Azubuike, Ayogu Thomas Eze, Onwa Ndubuisi Collins and Nwuzo Agabus Chidiebube

Three hundred sachet water samples produced in Enugu and Onitsha namely: Aqua Rapha, Bejoy, Teck Tack, SO and Rock Tama were collected from six different batches. They were screened for the presence of faecal coliforms using standard microbiology techniques. Sachet- water samples contaminated with faecal coliforms were selected and tested for their susceptibility to fourteen different antibiotics by disc diffusion method. 80 (26.6%) were contaminated with faecal coliforms as follows: Aqua Rapha 25 (41.6%) specifically (ESCHERICHA COLI, ENTEROBACTER and KLEBSIELLA spp.), Rock Tama 1 (1.6%) (E. COLI,), Bejoy 20 (33.3%) (E. COLI, ENTEROBACTER and KLEBSIELLA spp.) , Teck Tack 9 (15%) (E. COLI, ENTEROBACTER and KLEBSIELLA spp.), and SO 33 (55%) (E. COLI, ENTEROBACTER and KLEBSIELLA spp). Susceptibility studies showed that E. COLI was highly resistance to trimethroprim, sulphamethoxazole/trimethroprim, ampicillin, aztrenoman, ceftazidime, and cefpirome but susceptible to gentamicin, ciprofloxacin, cefoxitin and cefotaxime. ENTEROBACTER and KLEBSIELLA spp. were totally resistance to all the antibiotics tested. We report the presence of multi-drug resistance fecal coliform in the form of E. COLI, ENTEROBACTER and KLEBSIELLA spp. from sachet- water.