RAPD and SSR analysis of wild oats (Avena species) from North West frontier Province of Pakistan.

Abstract


Zahid Hanif1 , Zahoor A. Swati1 , Imtiaz Khan2 *, Gul Hassan2 , K. B. Marwat2 , sabz Ali3 and M. Ishfaq Khan2

Genetic diversity of 10 biotypes of wild oats (Avena species) which differ in certain morphological and physiological characters were analyzed using 9 Randomly Amplified Polymorphic DNA (RAPD) and 2 Simple Sequence Repeat (SSR) primer sets. RAPD and SSR primers revealed different levels of genetic polymorphisms among the various biotypes. Range of genetic distances estimated by using individual primers was 0 – 100 % but on an average genetic distances were in the range of 30 - 65% indicating that these biotypes were genetically very diverse and possess a high degree of polymorphism

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