Abstract

M .T. Bakare Odunola1*, I. S. Enemali1 , M. Garba1 and O. O. Obodozie2

Samples were extracted with dichloromethane and the organic layer evaporated to dryness. The residue was dissolved in methanol, and 25 µl aliquot injected onto the column. Tolbutamide was used as the internal standard for chlorpropamide. The UV detector response was linear over the range 0 – 300 µg/ml, with a correlation coefficient of 0.999 and detection limit of 1.30 ng/ml. Within day and betweenday assay variations was generally < 2.50%. No interference from endogenous constituent was observed. The utility of the method was demonstrated by determine chlorpropamide in samples from human volunteers following a single oral dose of 250 mg in drug interaction studies. The procedure is simple and fast, requiring small volumes of plasma.