Abstract

Omar ElBaradei Mubarak*, Naguib Faten Morsi and Mohamed Hamama

This study try to investigate the affirmation of 70% ethanol / water Hibiscus rosa extract (HRE), of the leaves, to alleviate the CCl4- induced hepatic antioxidants disorders and oxidative stress in male albino rats. For induction of early and late stages of liver intoxication, CCl4 (0.1ml/100gm b.w., twice a week, sc.) was used for 7 and 14 weeks respectively. At the end of both stages of intoxication, the diagnostic enzymes of liver function; serum aspartate transaminase (AST), and alanine transaminase (ALT) recorded highly significant elevation (p<0.001) with the two levels of intoxication while hepatic 5'-nucleotidase(5'-NT) recorded highly significant elevation (p<0.01)only with the late stage of intoxication. On the other hand, the hepatic antioxidants defense markers, reduced glutathione (GSH), glutathione S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), showed significant diminish, one as compared to its control. This emphasizes the increased oxidative stress in the liver with both early and late stages of CCl4-intoxication. Consequently, the oxidative stress biomarkers, lipid peroxidation, as malondialdehyde (MDA), liver xanthine oxidase (XO) and serum advanced oxidation protein products (AOPPs) significantly elevated (p<0.001) by CCl4 liver injury, in stage dependent manner. HRE Post-treatment (100 mg/kg b.w., orally /day, for 4 weeks) for rats, whose of liver injuries, significantly reduced the all alternations of liver functions, antioxidant defenses and oxidative stress- markers with time dependent manner. It was clear that both CCl4-induced alternation and HRE amelioration effects were of hepatotoxic stage-dependence. So, it is suggested that, H. rosa sinensis extract effectively ameliorated the changes observed with CCl4 injuries in liver, possibly through antioxidant and/or free radical scavenging effects of its phenolic compounds, specially flavonoids, triterpenes and tannins, which previously were demonstrated as a phytochemical components in this extract.