Abstract

Iroha I. R.*, ILang D. C., Ayogu T. E., Oji A. E. and Ugbo E. C.

Ethanol, hot and cold crude water extracts of five medicinal plants namely Vitex doniana (root) , Cassia tora (Leaf), Alstonia boonei (bark), Stachytarpheta jamaicensis (leaf), and Carica papaya (leaf) used as traditionally medicine for anti Salmonella typhi activity in Ebonyi state were evaluated. These plants were screened in-vitro for anti-typhoid activity against 10 clinically selected isolates of S. typhi using the hole-plate diffusion method. The Minimum inhibitory concentration (MIC) of Ethanol, hot and cold water extract of each herbal plant was determined by broth dilution method. Ethanol extracts of Vitex doniana exhibited anti-typhoid activity against 9(90%) of the test organisms, A. boonei exhibited activity against 8(80%) of the test organisms, C. papaya against 2(20%), C. tora against 6(60%), and S. jamaicensis against 6(60%). Hot water extract of Vitex doniana showed anti-typhoid activity against 7(70%) of the test organisms, A. boonei against 9(90%), C. papaya against 1(10%), C. tora against 8(80%) and S. jamaicensis against 7(70%). Cold water extract of V. doniana, had anti-typhoid activity against 6(60%) of the test organisms, A. boonei, against 6(60%), C. papaya against 0(0%), C. tora against 6(60%) and S. jamaicensis against 4(40%). MIC of ethanol, hot and cold water extracts of V. doniana, A. boonei, C. papaya, C. tora and S. jamaicensis, fall within 0.4 -128, 0.8 -128, 64 -128, 32 – 128 and 32 – 128. MIC of hot water extracts were within 16 -128, 0.8 – 128, 128 -512, 0.8 – 512 and 0.8 – 128 while MIC of cold water extract are within 64 – 128, 64 – 512, 64 – 512, 64 – 512 and 128-512 respectively. Our findings showed that ethanol and hot water extracts of V. doniana and A. boonei had the best anti-typhoid activity followed by C. tora and S. jamaicensis while C. papaya showed no activity.