Abstract

Faruk Hossain*, Golam Sorowar, Sharmin Akter, Mahbubul Hoque and Quazi M. Emdadul Huque

The study was conducted to find out the variation of semen quality among three successive graded up (F1, F2 and F3) Sahiwal × local breeding bulls for Artificial Insemination (AI) purposes. Total of 468 ejaculates were collected from 9 breeding bulls throughout the experimental year 2019. The recorded data were summarized using Microsoft Excel 2010 and statistically analyzed using GraphPad Prism 5 software. Out of 468 ejaculates 385 (82.26%) were found to be creamy in color followed by 64 (13.68%) and 19 (4.06%) as yellowish and watery, respectively. Level of up-gradation had significant (P<0.05) effect on ejaculate volume, consistency, mass activity, sperm concentration and initial and post motility. The highest (5.356 ± 0.10 ml) and the lowest (4.726 ± 0.09 ml) volume of semen were found in third (F3) and first (F1) crossing, respectively (p<0.05). The mass activity ranged from 3.216 ± 0.04 to 4.389 ± 0.05. Semen pH varied insignificantly but sperm concentration, initial motility and post freezing motility had significant differences (p<0.05). Initial and post motility ranged from 71.49 ± 0.24 % to 78.89 ± 0.43% and from 50.29 ± 0.39% to 53.46 ± 0.36%, respectively. It was concluded that most of the semen quality parameters were influenced by level of up-gradation and freezing. Semen characteristics were better in F3 followed by F2 group.