Abstract

Munish Garg*, Chanchal Garg, V. J. Dhar and A. N. Kalia

Alcoholic extract of Phyllanthus fraternus Webster whole plant (PFAE) prepared by successive solvent treatment was administered at a dose of 500 mg/kg body weight once in a day for 21 days to the alloxan induced diabetic albino rats. Certain biochemical parameters that is lipid profile (total cholesterol, high density lipoprotein, triacylglycerols), kidney functions (urea, creatinine) and liver functions (alkaline phosphate, alanine aminotransferase, aspartate aminotransferase) were evaluated and compared with normal and standard drug tolbutamide (200 mg/kg body weight) administered group. As a result, drug treatment has significantly improved the disturbed biochemical parameters at variable degrees when compared with standard drug. The phytochemical studies conducted for standardization of the extract showed the presence of tannins and flavonoids as major phytoconstituents. The total phenolics content was found to be 37.51 mg/g of drug extract. Quantitative estimation carried out on two major flavonoids by HPTLC confirmed a concentration of 1.706% w/w rutin and 5.614% w/w of quercetin present in the alcoholic extract. In conclusion, owing to the positive potential activity against disturbed biochemical parameters associated with diabetes, P. fraternus can be used effectively in the management of this deadly disease.