Abstract

Nian XIN, Wei LI, Yu-Juan LI, Xiao-Ke MA, Zhen-Ping FU and Yan LI*

Gaoreqing freeze-dried powder was effective fraction extracted from Lonicera japonica Thunb., Scutellaria baicalensis Georgi and Forsythia suspense (Thunb.) Vahl. It was confirmed to have certain anti-virus, antibacterial effects and immuno-enhancing activities. Cell culture and influenza viral pneumonia model of mouse were used to study its antiviral effect. Plate culture and pathogenic bacteria-infected model in mouse were established to examine its anti-bacterial effect. Carbon particle clearance test and determination of serum hemolysin were done to observe its immune-enhancing effect. The results showed that Gaoreqing had significant effect on inhibiting cytopathic effect induced by various viruses. Compared with virus control group, high and middle dosage groups could significantly decrease the pulmonary index, pulmonary virus hemagglitination titer in influenza viral pneumonia model of mouse (P < 0.01). It showed obvious inhibitory effects on Streptococcus pyogenes, Staphylococcus aureus, pneumococcus and so on. Gaoreqing also had obvious protective effect on mice infected by S. aureus and pneumococcus. In addition, phagocytosis and 50% hemolyzing concentration of mice were obviously reinforced by Gaoreqing (P < 0.05 to 0.01). The results obtained here demonstrated that Gaoreqing had obvious anti-virus and anti-bacterial effects and enhanced immune function in mice