Abstract

Yi Nan1, Ling Yuan2,3, Li Zhou2and Yang Niu1

The objective of this research is to study the technology for the extraction of flavone in Scutellariabaicalensis and its antibacterial activity; to adopt macroporous absorption resin method to investigatethe technology for purification of total flavone in S. baicalensisthrough orthogonal experiment, and adopt the improved tube double dilution method to study its antibacterial activity. The final determined optimal purification technology is A2B1C3, that is, the resin column diameter-height ratio is 1:5,eluent flow rate is 0.5 ml/min, and eluent consumption is 9BV. The average content of total flavone purified by resin reaches 89.67%. The MIC of this extract to Staphylococcus aureusand Klebsiella pneumoniais 1.56 and 3.13 mg/ml respectively. Macroporous absorption resin technology is the efficient method to extract flavone in S. baicalensis, which has strong antibacterial activity