Study on ultrastructure of Leishmania major and Lizard leishmania.

Abstract


Bahram Kazemi , Mohammad Hassan Heidari , Mehdi Naderi , Abbas Piryaei and Mohammad -Reza Nazari -Pouya

We compared ultrastructure of Lizard Leishmania and Leishmania major promastigotes by electron microscope. Leishmania major (MRHO/R64/Nadim1) and Lizard leishmania promastigotes were mass cultured, collected by centrifugation and washed by PBS buffer three times, the ultrastructural features of both Leishmania promastigotes were observed by transmission electron microscope Their subpellicular microtubules diameter and cytoplasmic membrane thickness are identical, but there is difference in two parasites in respect of distance between kinetoplast and flagella. We could not find golgi apparatus in Lizard Leishmania.

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