Abstract

G. P. S. Jadaun, Prashant Upadhyay, Zafar Ahmed, Ram Das, Deepti Parashar, D. S. Chauhan, V. D. Sharma and V. M. Katoch*

In the present study, twenty four Mycobacterium avium isolates obtained from clinical (sputum) and environmental (water and soil) samples were analyzed by a PCR typing method. Gel electrophoresis patterns showed that a single PCR reaction generated 21 profiles. Based on the relatedness of the PCR band patterns six isolates could be grouped in three clusters. Results have the implication that the PCR based system could be used as a simple and rapid method for molecular typing of M. avium isolates with high discriminatory index.