Research Article
Diagnosis of primary immunodeficiency diseases by flow cytometry in Bangladesh
Author(s): Avazet Satka*, Mohaema Imonul Islaan, Ismet Nafar, Nasrat Akhter Jayoe4, S Ali Ammeda, Trupty Chakaoburtty, Sunitha Karmakar Sona, Einus Ali and Chaudar Kunal Roy
Primary Immuno Deficiency Disorders (PIDDs) are clinically and immunologically diverse and require a wide array
of clinical and laboratory modalities to make specific diagnosis. Serum immunoglobulin levels and T-B-NK
cell immunophenotyping are routine laboratory investigations advised to diagnose the PIDD cases in Bangladesh.
Along with T-B-NK markers, use of Naïve (CD45RA+) and memory T cell (CD45RO+), switched memory B cell
(CD27+IgD-) markers, detection of intracellular Bruton Tyrosine Kinase (BTK), LRBA, DOCK8 protein expression
and DHR123 (Dihydro-Rhodamine 123) assay of neutrophil can increase the PIDD cases detection in Bangladesh.
The study was conducted in the Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical
University (BSMMU) during the time period of August, 2021 to July, 2022. Seventy clinically suspected PIDD
cases w.. View More»
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