Emilande Guichet

Department of Molecular and Cellular Biology, University of Douala, Douala, Cameroon

Publications

• Research Article   
  First identification of ba.5.2.1 omicron sub-lineages in fully vaccinat- ed patients in Cameroon
  Author(s): Martin Maïdadi Foudi, Célestin Godwe, Emilande Guichet, Nicolas Fernandez Nuñez, Guy-Roland Awoundza, Nadine Lamare, Marie Amougou Atsama, Marcel Tongo, Onana Boyomo, Wifred Mbacham, Ahidjo Ayouba, Martine Peerters, Eric Delaporte and Charles Kouanfack*
  
  Omicron is the most mutated SARS-CoV-2 variant; its high transmissibility and immune evasion ability have raised global concerns. We reported here the first identification of BA.5.2.1 Sub-lineage of Omicron variant in two fully vaccinated patients in Cameroon. We used DICOV-MOC/I MULTIPLEX RT-PCR and CovidSeq on Illumina ISeq100 respectively for the screening of SARS-CoV-2 mutations and whole-genome sequencing. We detected antibodies to Spike (SP) and Nucleocapsid (NC) proteins from ancestral and Omicron SARS-COV-2 strains using a multiplex Luminex-based assay on a MagPix platform. On the 15th of June 2022, two patients (Cases 1 and 2) presented at CREMER laboratory in Yaoundé for COVID-19 test. Nasopharyngeal Swab RT-PCR results were positive with Treshold Cycle of 16.7 and 19.3 for both the cases respectively. Mutations screening revealed the presence of K417N and L452 mutati.. View More»
  

  Abstract HTML PDF