Nicolas Fernandez Nuñez

Publications

• Research Article   
  First identification of ba.5.2.1 omicron sub-lineages in fully vaccinat- ed patients in Cameroon
  Author(s): Martin Maïdadi Foudi, Célestin Godwe, Emilande Guichet, Nicolas Fernandez Nuñez, Guy-Roland Awoundza, Nadine Lamare, Marie Amougou Atsama, Marcel Tongo, Onana Boyomo, Wifred Mbacham, Ahidjo Ayouba, Martine Peerters, Eric Delaporte and Charles Kouanfack*
  
  Omicron is the most mutated SARS-CoV-2 variant; its high transmissibility and immune evasion ability have raised global concerns. We reported here the first identification of BA.5.2.1 Sub-lineage of Omicron variant in two fully vaccinated patients in Cameroon. We used DICOV-MOC/I MULTIPLEX RT-PCR and CovidSeq on Illumina ISeq100 respectively for the screening of SARS-CoV-2 mutations and whole-genome sequencing. We detected antibodies to Spike (SP) and Nucleocapsid (NC) proteins from ancestral and Omicron SARS-COV-2 strains using a multiplex Luminex-based assay on a MagPix platform. On the 15th of June 2022, two patients (Cases 1 and 2) presented at CREMER laboratory in Yaoundé for COVID-19 test. Nasopharyngeal Swab RT-PCR results were positive with Treshold Cycle of 16.7 and 19.3 for both the cases respectively. Mutations screening revealed the presence of K417N and L452 mutati.. View More»
  

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