Samuel Onyoyo Nguya

Department of Biotechnology, KALRO Biotechnology Research Institute Kikuyu, Nairobi, Kenya

Publications

• Research   
  A multi-epitope-based subunit vaccine candidate against peste des petit generated through reverse vaccinology
  Author(s): Willis A Adero*, Steven Nyanjom, Martin Kiogora Mwirigi, Yattinder B, Samuel Onyoyo Nguya and Paul Kimutai kirui
  
  Peste des Petits Ruminant Virus (PPRV) is an acute, highly contagious viral disease of small ruminants, endemic to su-Saharan Africa, Asia and the Arabian Peninsula. The disease is a major constraint to food security, causing significant economic losses to subsistence farmers in affected areas. The fusion and Hemagglutinin proteins are highly immunogenic and produced in large quantities in virus-infected cells. This makes them suitable targets for the host’s immune response. This study aimed at developing and evaluating effective multi-epitope vaccine candidate against PPRV developed from two immunogenic proteins Hemagglutinin (H), and Fusion (F), using a bioinformatics approach. In order to construct the subunit vaccine candidates, plasmid pET28a (+) was used as an expression vector, which was later transformed into E. coli DH5-α cells, followed by plasmid extrac.. View More»
  

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